Quantitative analysis of samples for Neogen's Histamine (Life Science Format) kit (409010).
If accounting for substrate background define a blank group on the layout. The average of these absorbance values are subtracted from other samples.
This method calculates the maximal binding (%B/B0) of each standard by dividing the absorbance of the standard by the B0 absorbance and multiplying by 100. The %B/B0 is plotted against concentration and a Four Parameter Logistic (4PL) curve if fitted through the points. Concentrations of samples are interpolated from the curve with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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