Quantitative analysis of samples for Neogen's Histamine (Life Science Format) kit (409010). If accounting for substrate background define a blank group on the layout. The average of these absorbance values are subtracted from other samples. This method calculates the maximal binding (%B/B0) of each standard by dividing the absorbance of the standard by the B0 absorbance and multiplying by 100. The %B/B0 is plotted against concentration and a Four Parameter Logistic (4PL) curve if fitted through the points. Concentrations of samples are interpolated from the curve with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s 11-d-Thromboxane B₂ (408010) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s 11ß-Prostaglandin F_2a (404410) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s 13,14-dihydro-15-Keto-Prostaglandin F_2a (404610) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s 15-EPI-Lipoxin A₄ (407110) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s 6-keto-Prostaglandin F_1a (404310) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s Androstenedione (402010) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s Corticosterone (402810) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s Cortisol (402710) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s Cyclic AMP (403110) kit. This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements. The maximal binding (%B/B0) is calculated for each sample using the B0 measurement. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.