This data analysis tool calculates sample concentrations using linear regression in accordance with Neogen’s Creatinine (430210) kit.
Provide your data as the difference between the two sets of readings. I.e. if your microplate reader does not do these subtractions manually, then subtract the second set of absorbance readings from the first and provide the differences.
The standard data points (concentration vs. OD) are plotted on linear axes and linear regression is used to find the best straight line through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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