Cisbio HTRF Mouse IL1 alpha

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Detection of mouse IL1 alpha using HTRF (an alternative to ELISA) Cisbio kit 62MIL1APEG.

Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation. Cisbio's Mouse IL1 alpha kit is a homogeneous, add and read assay to measure IL1 alpha secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes