Detection of mouse CCL2 using HTRF (an alternative to ELISA) Cisbio kit 62MCCL2PEG.
Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages and dendritic cells. Its main role is to attract monocytes, basophils and T cells. CCL2 is involved in pathologies involving monocytic infiltrates like psoriasis or atherosclerosis. In the brain, CCL2 is released by neurons, astrocytes and microglia and is part of the neuroinflammatory response observed in epilepsy, ischemia or Alzheimer’s disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to Diabetes. Cisbio's Mouse CCL2 kit is a homogeneous, add and read assay to measure CCL2 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:
1. Delta Ratio is computed for each sample using the zero standard (Zero).
2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
3. The %CV and Standard Deviation are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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