Cisbio HTRF Human TNF alpha

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Cisbio

Detection of human TNF alpha using HTRF (an alternative to ELISA) Cisbio kit 62HTNFAPEG.

Also called CXCL8, TNF alpha is a chemokine mainly produced by macrophages, T cells and neutrophils. TNF alpha acts as a chemoattractant for neutrophils, and promotes infiltration and activation at inflammation sites. TNF alpha is involved in several cancer types for its ability to promote angiogenesis and cell proliferation, as well as inhibit apoptosis. Cisbio's Human TNF alpha kit is a homogeneous, add and read assay to measure TNF alpha secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes