Cisbio HTRF Human TGFB1

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Cisbio

Detection of human TGFB1 using HTRF (an alternative to ELISA) Cisbio kit 62HTGFBPEG.

Also known as Transforming growth factor, beta 1 ; Gene name TGFB1 ; Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes