Detection of human IL2 using HTRF (an alternative to ELISA) Cisbio kit 62HIL02PEG.
IL2 major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes the differentiation of T cells into effector or memory T cells thus helping the body fight off infection. IL2 is also a key player in immuno-oncology since it is the main promoter of the differentiation of T cells into Treg. Cisbio human IL2 kit is a homogeneous, add and read assay to measure IL2 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:
1. Delta Ratio is computed for each sample using the zero standard (Zero).
2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
3. The %CV and Standard Deviation are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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