Cisbio HTRF Human IL17

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Cisbio

Detection of human IL17 using HTRF (an alternative to ELISA) Cisbio kit 62HIL17PEG.

IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of the response to fungi and bacteria, and promotes the release of other pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and auto-immune diseases like asthma, psoriasis or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is the main cytokine released by differentiated TH17 cells. Cisbio's Human IL17 kit is a homogeneous, add and read assay to measure IL17 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes