Detection of human IL1 alpha using HTRF (an alternative to ELISA) Cisbio kit 62HIL1APEG.
Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation. Cisbio's Human IL1 alpha kit is a homogeneous, add and read assay to measure IL1 alpha secretion in supernatant. It offers a fast, efficient alternative to ELISA.
This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:
1. Delta Ratio is computed for each sample using the zero standard (Zero).
2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
3. The %CV and Standard Deviation are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
Only members can access this function.
Please log in or join now (it's free to join).