Detection of human G-CSF using HTRF (an alternative to ELISA) Cisbio kit 62HGCSFPEG.
Also known as CSF3 (colony-stimulating factor 3), G-CSF is a hematopoietic cytokine. It acts on precursor cells in the bone marrow to initiate their proliferation and differentiation into mature granulocytes, on hematopoietic stem cells to induce their mobilization in the blood stream, and on neurons to promote neurogenesis and block apoptosis. In myocardial infarction or ischemia, G-CSF promotes T cells' immune tolerance and tissue recovery. The absence of a functional G-CSF receptor is associated with congenital neutropenia. Cisbio's Human G-CSF kit is a homogeneous, add and read assay to measure G-CSF secretion in supernatant. It offers a fast, efficient alternative to ELISA.
This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:
1. Delta Ratio is computed for each sample using the zero standard (Zero).
2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
3. The %CV and Standard Deviation are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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