Cisbio HTRF Human CCL5

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Detection of human CCL5 using HTRF (an alternative to ELISA) Cisbio kit 62HCCL5PEG.

Also known as Rantes (regulated upon activation, normal T-cell expressed and secreted), CCL5 is a chemokine mainly secreted by T lymphocytes, macrophages, platelets, synovial fibroblasts, tubular epithelium and certain types of tumor cells. CCL5 plays an important role on inflammatory sites by recruiting T cells, macrophages, eosinophils and basophils. When secreted with IL-2 and IFN-g, CCL5 also induces the activation and proliferation of natural killer cells.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes