Cisbio HTRF Human CCL3

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Cisbio

Detection of human CCL3 using HTRF (an alternative to ELISA) Cisbio kit 62HCCL3PEG.

Also called MIP 1 alpha (macrophage inflammatory protein 1 alpha), CCL3 is a member of the MIP (Macrophage Inflammatory protein) also including CCL4 (MIP1 beta). This chemokine is secreted by monocytes, macrophages, T cells and endothelial cells. CCL3 plays an important role on the activation of the inflammatory response by attracting monocyte-lineage cells and lymphocytes to inflammation sites. CCL3 has been reported as a stem cell inhibitory factor. Cisbio's Human CCL3 kit is a homogeneous, add and read assay to measure CCL3 secretion in cell supernatant. It offers a fast and efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes