Detection of human CCL20 using HTRF (an alternative to ELISA) Cisbio kit 62HCC20PEG.
Also called LARC (liver activation-regulated chemokine) or MIP3A (macrophage inflammatory protein 3), CCL20 is a chemokine mainly present in peripheral blood lymphocytes, lymph nodes and liver. CCL20 secretion is upregulated by microbial factors and inflammatory cytokines like TNFa or IFNg. Its main role is to attract lymphocytes and dendritic cells to the site of infection. In CNS, CCL20 released by endothelial cells has been shown to attract TH17 cells and promote their infiltration to the lumbar spinal cord. Cisbio's Human CCL20 kit is a homogeneous, add and read assay to measure CCL20 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:
1. Delta Ratio is computed for each sample using the zero standard (Zero).
2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
3. The %CV and Standard Deviation are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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