Quantitative determination of alkaline phosphatase (ALP) present in a variety of samples. Data analysis is performed in accordance with Arbor Assays' DetectX Alkaline Phosphatase Colorimetric Activity Kit (K082-H1).
The standard data points are plotted (concentration vs. OD) and a Four Parameter Logistic Fit is made through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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