WorkOut Plus Video Guides

Information for system-administrators for performing a new installation of WorkOut Plus. Topics include: • Installing WorkOut Plus (example given on Windows 7) • Using WorkOutPlusWiz2Config tool • Inserting USB dongle

Information for system-administrators for upgrading an existing WorkOut 2.x installation to WorkOut Plus. Topics include: • Uninstalling WorkOut 2.x • Transferring WorkOut 2.x data files (including, protocol, results and layouts) to WorkOut Plus • Installing WorkOut Plus • Using WorkOutPlusWiz2Config tool • Inserting USB dongle

Overview of the interaction between WIZARD² software and WorkOut Plus. Explains how WorkOut can be used to process data by a manual import or automatic launching (via the WIZARD² software).

A top-level view of WorkOut, with an explanation and examples of key data key concepts. Topics covered include: • Protocol files • Results files • Matrices • Layouts • Edit Layout • Edit Transforms • Sample IDs • Transforms • Report Content

How to configure a WorkOut Plus protocol to calculate %CV and % bindings (%B/B0). Topics include: • Creating a new protocol • Importing CSV file • Sample Types • Edit Types • Adding Custom Types • Pos Control • Neg Control • Calculating %CV • Calculating %B/B0 • Matrix Expression • Single Cut-off transform

How to use the installed example protocols to quickly get started with WorkOut data analysis. Topics covered include: • Introduction to the provided example files • Configuring a protocol to automatically print • Duplicating protocols • Changing layout/browsing layout • Setting standard concentrations

How to create a WorkOut assay protocol file for RIA. Topics covered include: • Creating a new protocol from scratch • Manually importing CSV file • Creating a rack layout • Normalize • Standard Curve Fit • Selecting Fit Method • Using Four Parameter Fit • Numerical Formatting for decimal places/significant figures • Showing replicates or average in report • Save Protocol • Running protocol

Using WorkOut's layout editor when working with racks. Topics include: • Create a new layout in a new protocol • Defining replicate groups • How WorkOut automatically configures further racks according to the defined layout • Add Rack button • Changing the layout after analysis • Changing a protocol's layout • Reading a control after a sequence of unknowns

How to use WorkOut to generate inter-assay quality control charts and reports. Topics include: • How data is arranged in WorkOut • Monitoring observations • Levey-Jennings, CUSUM, observation table • Export QC report to Excel • Creating QC reports on- the-fly with Create Now • Monitoring Control Concentration • Monitoring R² (goodness of fit)

Introducing WorkOut acceptance rules for intra-assay validation. Topics include: • Creating rules to automatically check the assay was prepared correctly. • Intra-assay validation • Validation Transform • Validation Expression transform • %CV calculation

Continuing the explanation of acceptance rules with a focus on inter-assay rules. Topics include: • Validating with absolute range • isinrange function • Inter-assay validation • Calculating standard deviation from all QC observations • isinpmrange function • Referring to monitored QC observations (with QC1, QC2, etc.) • How to find more information about QC expression functions and examples • Westgard rules

Provides an overview of how WorkOut data files are protected when using Enhanced Security. Topics include: • Protected folders • Restricted operations • Parent Directory for data files • Secure file operations

Information for system administrators for administering WorkOut Plus. Topics include: • Authentication and Authorization • Administering users with Windows User Management • Creating WorkOut users • WizUsers group • Setting password policy for WorkOut users • Changing user passwords • Logging in to WorkOut Plus • Using WorkOut Security Management console • Setting a user’s authorization group • Creating custom authorization group • Accessing exporting and printing audit trail • WorkOut anonymous account and automatic log in for routine use

Information for general users working with WorkOut Enhanced Security with WIZARD². Topics include: Key features of Enhanced Security • Protected data area, authorization, authentication, user levels • Anonymous account for automatic analysis • Audit trail overview • Logging in • Time out period • Log out • Logging in to separate windows • Auto Log out • Time out log • Authentication re-use

A closer look at WorkOut's powerful expression based features. Topics include: • Normalize Values • % Binding • Referring to sample groups on the layout • Expression Transform • Referring to Control1 • Evaluation by Layout • Flagged positions • Matrix Expression Transform • Dual Matrix Expression (using x and y) • List operations • Highlight Transform • Inserting a Transform • Auto Flag Transform • Calculating %CV • Boolean operations • Where to find more information

Using Worklists with WorkOut. Topics include: • Importing data from a file into a WorkOut report • Importing sample IDs from a text file • Configuring Worklist • Using a specific file or using the newest file in a folder • Worklist formats (Text and CSV) • Running a protocol with Worklist feature • Importing standard concentrations and dilution factors in a worklist

Creating a custom export format using the Custom Text Export transform. Topics include: • Using the Custom Export transform • Generating the text file • Custom Export configuration files • Using the Export Configuration Editor • ExportingConfig XML • Referencing GLP features • Evaluating expressions in the export • Specifying transform outputs • Configuring the text export output

How to create a WorkOut Plus protocol that calculates concentrations using standards measured in previously ran assay. Topics include: • Duplicating a results file • Importing Tri-Carb CSV file • Select Measurements from Previous Assay • Edit Layout • Quantitative protocol without standards on layout • Save Protocol

How to improve fit when using a zero value standard with the 4PL (Four Parameter Logistic Fit). Topics include: • Example problem data • Resolving with a suitably small concentration value • Resolving by fixing asymptote • Add a Zero type • Changing layout • 4PL coefficients • 4PL asymptotes

How to calculate Plasma Renin Activity (PRA) in WorkOut Plus. Topics include: • PRA Calculation • Layout Editor • Matrix Expression • Save Protocol