Competitive immunoassay for the quantitative measurement of estradiol (pg/mL).
Calculations are made by subtracting the mean of the NSB measurements from the provided optical densities. The B/B0 is calculated from the corrected values using the mean of the B0 group. Concentrations are calculated using a Four Parameter Logistic Fit (4PL) and dilution factors are applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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