Human uPA Total Antigen

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Molecular Innovations

Antigen assay for the quantitative determination of total urokinase plasminogen activator in human plasma, serum, urine, cell culture media, or tissue extracts. This data analysis tool calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Molecular Innovations' Human uPA Total Antigen ELISA Kit.

The standard data points are plotted (concentration vs. absorbance) on linear axes and a 4PL curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

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Measurements

Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.
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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes