Antigen assay for the quantitative determination of total urokinase plasminogen activator in human plasma, serum, urine, cell culture media, or tissue extracts. This data analysis tool calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Molecular Innovations' Human uPA Total Antigen ELISA Kit.
The standard data points are plotted (concentration vs. absorbance) on linear axes and a 4PL curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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