Enzyme immunoassay (EIA) for the quantification of insulin in mouse and rat sera.
A dose response curve of the corrected absorbance (450nm - 590nm) vs. concentration is generated using a 5-parameter logistic fit. Concentrations of insulin, present in the samples, are determined directly from this curve with any specified dilution factors applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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