Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit to compute concentration values for the Hycult Biotech Human LL-37 kit.
This analysis first calculates the mean absorbance for each group of sample replicates. The standard (concentration vs. measurement) are plotted on semi-log axes and a 5PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
Samples with an absorbance greater than the highest standard are highlighted in yellow. Hycult Biotech recommends these samples are diluted and re-assayed. The analysis also verifies that the mean absorbance of the zero standard is less than 0.3. Also, it checks that the %CV of all samples is <15.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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