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Quantitative analysis of samples using a Four Parameter Logistic Fit (4PL) with 1/y2 weighting weighting. The applied weighting is used to offset heteroscedasticity by taking into account the change in variance which occurs with an increase in signal.
All samples are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected measurement) and a Four Parameter Logistic Fit (4PL) with 1/y2 weighting is made through these points. The concentrations of the unknown samples are determined from the fit. It is important to note that concentrations can only be determined for samples which fall within the range of the determined upper and lower asymptotes of the fit (the a and d parameters).
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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