Determines titers of antibodies generated by immunization. In some samples there is a cross-reacting, non-specific antibody present in the serum before immunization (pre-immune Serum). This non-specific signal is subtracted out using a pool of non-immune serum. Corrected Antibody dilution curves are calculated for each unknown and used to find the dilution at a specified % of Pos Control.

Calculates IC50 values of samples measured at serial dilutions using the Four Parameter Fit method. Fits are overlaid for comparison.