In vitro quantitative assay for detecting Peptide YY based on the principle of competitive enzyme immunoassay. Data analyis is performed in accordance with Sigma-Aldrich's Peptide YY EIA Kit. % Absorbance is calculated for all samples from the Blank and B0 sample measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic (4PL) curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

In vitro quantitative assay for detecting Ghrelin peptide based on the principle of competitive enzyme immunoassay. Data analyis is performed in accordance with Sigma-Aldrich's Ghrelin EIA Kit. % Absorbance is calculated for all samples from the Blank and B0 sample measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic (4PL) curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

In vitro quantitative assay for detecting GLP-1 peptide based on the principle of competitive enzyme immunoassay. Data analyis is performed in accordance with Sigma-Aldrich's GLP-1 EIA Kit. % Absorbance is calculated for all samples from the Blank and B0 sample measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic (4PL) curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

For the quantitative determination of SOD activity and concentration. This method performs analysis in accordance with the Sigma-Aldrich SOD Determination Kit (19160).