Quantitative analysis of samples using a Four Parameter Logistic (4PL) curve fit in accordance with MaxSignal® Salbutamol ELISA test kit. This analysis constructs a standard curve by plotting the mean relative absorbance from each standard against its concentration in ng/mL on a semi-logarithmic curve. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.