Enzyme linked immunosorbent assay (ELISA) for the detection of Dog IgG1 in serum or plasma. This method first subtracts the average OD of the blank wells from all other measurements. A standard curve is plotted and the Four Parameter Logistic Fit is performed. Concentration values are calculated from the curve and the specified dilution factors are applied.

Enzyme linked immunosorbent assay (ELISA) for the detection of Mouse Albumin in serum, plasma or urine in accordance with Bethyl Laboratories Mouse Albumin ELISA Quantitation Set. This analysis optionally includes a background correction step. If a blank group is included on the layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a Four Parameter Logistic (4PL) curve is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Enzyme linked immunosorbent assay (ELISA) for the detection of pig IgA in serum, plasma, colostrum and milk in accordance with Bethyl Laboratories Pig IgA ELISA kit. (Other biological samples, such as saliva, tears, nasal and intestinal secretions, may be suitable samples.) This analysis optionally includes a background correction step. If a blank group is included on the layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a Four Parameter Logistic (4PL) curve is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.