Assay Layout Definition

The assay layout defines how your samples are arranged (on a microplate or rack).

Here is an example of an assay layout for a 96-well microplate:

The colour identifies the sample type and the number identifies the group for each position. Together the type colour and group number uniquely identify each measured sample. Positions with the same colour and number are replicates of the same sample. Wells not used appear as empty white wells (such as H1 and H2).

In the layout shown above, positions A1 and A2 are replicates of Standard1, there is 1 Blank group and 40 Unknowns, with all samples in duplicate.

Most assays allow you to specify your own layout. You can freely position your samples and change the number of replicates providing the requirements of the assay are met. Further information is available on creating your own assay layout.

Certain assay pages allow you to provide measurement data ran across multiple plates.

The numbering of samples is contiguous and sequential for each type. When defining a layout for multiple plates, group numbering of samples should start at 1 for each plate. Numbering each group on each from 1 plate makes it easier to define multiple plate layouts.